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Identification and characterization of cis-regulatory elements 'insulator and repressor' in PPARD gene.
Yadav, Dilip K; Shrestha, Smeeta; Dadhwal, Gunjan; Chandak, Giriraj R.
Afiliação
  • Yadav DK; Genomic Research on Complex Diseases (GRC Group), CSIR-Centre for Cellular & Molecular Biology, Hyderabad, Telangana, 500 007, India.
  • Shrestha S; Genomic Research on Complex Diseases (GRC Group), CSIR-Centre for Cellular & Molecular Biology, Hyderabad, Telangana, 500 007, India.
  • Dadhwal G; Building No.7, School of Basic & Applied Sciences, Dayananda Sagar University, Shavige Malleshwara Hills, Kumaraswamy Layout, Bangalore 560 078, Karnataka, India.
  • Chandak GR; Genomic Research on Complex Diseases (GRC Group), CSIR-Centre for Cellular & Molecular Biology, Hyderabad, Telangana, 500 007, India.
Epigenomics ; 10(5): 613-627, 2018 05.
Article em En | MEDLINE | ID: mdl-29583017
AIM: Identification and functional characterization of cis-regulatory elements in human PPARD gene. METHODS: We used various bioinformatic tools on the publicly available human genome and Encyclopedia of DNA Elements databases to explore potential cis-regulatory elements in PPARD gene region. RESULTS: We predicted an insulator and an enhancer element in intron 2 of PPARD gene. Functional characterization using transient transfection, reporter assay and CTCF binding confirmed the insulator status. However, the predicted enhancer element showed repressor/silencer activity. Finally, we observed a potential interaction between these two cis-regulatory elements which is in agreement with 5C-Encyclopedia of DNA Elements data. CONCLUSION: We report two functionally validated cis-regulatory elements in PPARD gene which will aid in understanding its regulation and role in metabolic functions.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Genoma Humano / Elementos Facilitadores Genéticos / Elementos Isolantes / PPAR delta Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Genoma Humano / Elementos Facilitadores Genéticos / Elementos Isolantes / PPAR delta Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article