Beta-3 adrenoceptor expression in the uterosacral ligament in the postmenopausal women with pelvic organ prolapse.
Neurourol Urodyn
; 37(7): 2135-2140, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-29635716
ABSTRACT
AIMS:
ß3-adrenoceptor (ß3-AR) agonist is known to relax the detrusor muscles. Considering that both pelvic organ prolapse (POP) and overactive-bladder (OAB) are frequently seen in the elderly population, the effect of ß3-AR agonist on the pelvic floor tissues should be investigated. As a first step, this pilot, experimental, and descriptive study was designed to investigate the presence of ß3-AR expression in the uterosacral-ligaments (USLs) in postmenopausal (PMP) women with POP.METHODS:
After informed consent, PMP women undergoing hysterectomy for POP were recruited for the intraoperative collection of distal USL between 5/2016 and 8/2016. H&E analysis for the USLs and immunohistochemistry (IHC) analysis for ß3-AR and α-actin smooth muscle (SM) were carried out. Tissue composition and percentage of ß3-AR expression in the USLs were subjectively measured and the staining results were expressed as descriptive analysis.RESULTS:
Total 11 USL specimens were collected and nine specimens were included for the final analysis (one specimen used for antibody concentration adjustment and one specimen failed proper staining). Under high-power-field, the USLs were composed of SM cells (81.5 ± 7.47%) and connective tissues (16.5 ± 7.9%). On IHC analysis, six out of nine specimens expressed ß3-AR with a different level of expression in SM cells in the USLs.CONCLUSIONS:
The majority of distal USLs were composed of SM cells. ß3-ARs are expressed in 67% of the study specimens. Considering that both POP and OAB are frequently seen in the elderly population, the effect of ß3-AR agonist on the pelvic floor tissues should be investigated further.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Região Sacrococcígea
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Útero
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Receptores Adrenérgicos beta 3
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Prolapso de Órgão Pélvico
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Ligamentos
Limite:
Aged
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Female
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article