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Quantitative evaluation of ruminal methane and carbon dioxide formation from formate through C-13 stable isotope analysis in a batch culture system.
He, Z X; Qiao, J Y; Yan, Q X; Tan, Z L; Wang, M.
Afiliação
  • He ZX; 1Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125,P.R. China.
  • Qiao JY; 1Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125,P.R. China.
  • Yan QX; 1Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125,P.R. China.
  • Tan ZL; 1Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125,P.R. China.
  • Wang M; 1Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125,P.R. China.
Animal ; 13(1): 90-97, 2019 Jan.
Article em En | MEDLINE | ID: mdl-29644945
Methane produced from formate is one of the important methanogensis pathways in the rumen. However, quantitative information of CH4 production from formate has been rarely reported. The aim of this study was to characterize the conversion rate (CR) of formic acid into CH4 and CO2 by rumen microorganisms. Ground lucerne hay was incubated with buffered ruminal fluid for 6, 12, 24 and 48 h. Before the incubation, 13C-labeled H13COOH was also supplied into the incubation bottle at a dose of 0, 1.5, 2.2 or 2.9 mg/g of DM substrate. There were no interactions (P>0.05) between dose and incubation time for all variables evaluated. When expressed as an absolute amount (ml in gas sample) or a relative CR (%), both 13CH4 and 13CO2 production quadratically increased (P<0.01) with the addition of H13COOH. The total 13C (13CH4 and 13CO2) CR was also quadratically increased (P<0.01) when H13COOH was added. Moreover, formate addition linearly decreased (P<0.031) the concentrations of NH3-N, total and individual volatile fatty acids (acetate, propionate and butyrate), and quadratically decreased (P<0.014) the populations of protozoa, total methanogens, Methanosphaera stadtmanae, Methanobrevibacter ruminantium M1, Methanobrevibacter smithii and Methanosarcina barkeri. In summary, formate affects ruminal fermentation and methanogenesis, as well as the rumen microbiome, in particular microorganisms which are directly or indirectly involved in ruminal methanogenesis. This study provides quantitative verification for the rapid dissimilation of formate into CH4 and CO2 by rumen microorganisms.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rúmen / Dióxido de Carbono / Microbioma Gastrointestinal / Formiatos / Metano Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rúmen / Dióxido de Carbono / Microbioma Gastrointestinal / Formiatos / Metano Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article