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Systematic forensic toxicological analysis by liquid-chromatography-quadrupole-time-of-flight mass spectrometry in serum and comparison to gas chromatography-mass spectrometry.
Grapp, Marcel; Kaufmann, Christoph; Streit, Frank; Binder, Lutz.
Afiliação
  • Grapp M; Forensic Toxicological Laboratory, University Medical Center Göttingen, Georg-August-University, 37075 Göttingen, Germany. Electronic address: mgrapp@med.uni-goettingen.de.
  • Kaufmann C; Forensic Toxicological Laboratory, University Medical Center Göttingen, Georg-August-University, 37075 Göttingen, Germany.
  • Streit F; Institute for Clinical Chemistry/UMG-Laboratories, University Medical Center Göttingen, Georg-August-University, 37075 Göttingen, Germany.
  • Binder L; Institute for Clinical Chemistry/UMG-Laboratories, University Medical Center Göttingen, Georg-August-University, 37075 Göttingen, Germany.
Forensic Sci Int ; 287: 63-73, 2018 Jun.
Article em En | MEDLINE | ID: mdl-29649771
Comprehensive screening procedures for psychoactive agents in body fluids are an essential task in clinical and forensic toxicology. With the continuous emergence and adaption of new psychoactive substances (NPS) keeping a screening method up to date is challenging. To meet these demands, hyphenated high-resolution mass spectrometry has gained interest as extensive and expandable screening approach. Here we present a comprehensive method for systematic toxicological analysis of serum by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS) with data independent acquisition. The potential of this method was demonstrated by analysis of 247 authentic serum- and 12 post-mortem femoral blood samples. Thus 950 compounds, comprising 185 different drugs and metabolites could be identified. For the detected substances, including pharmaceutical substances, illicit drugs as well as NPS, serum concentrations were confirmed ranging from traces to toxic values indicating the capability for forensic toxicological requirements. Positive identification of drugs was achieved by accurate mass measurement (±5ppm for [M+H]+; ±10ppm for [M-H]-), retention time (±0.35min), isotopic pattern match (less than 10 m/z RMS [ppm]), isotope match intensity (less than 20% RMS) and the presence of at least two fragment ions. The LC-QTOF-MS procedure was shown to be superior to serum screening by GC-MS, since 240% (335 versus 141) more drugs were identified in serum samples compared to GC-MS.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Preparações Farmacêuticas / Drogas Ilícitas / Cromatografia Líquida / Cromatografia Gasosa-Espectrometria de Massas Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Preparações Farmacêuticas / Drogas Ilícitas / Cromatografia Líquida / Cromatografia Gasosa-Espectrometria de Massas Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article