Lymphatic exosomes promote dendritic cell migration along guidance cues.
J Cell Biol
; 217(6): 2205-2221, 2018 06 04.
Article
em En
| MEDLINE
| ID: mdl-29650776
ABSTRACT
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified >1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Movimento Celular
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Vasos Linfáticos
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Exossomos
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Animals
/
Humans
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Male
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article