Your browser doesn't support javascript.
loading
Taraxasterol Inhibits LPS-Induced Inflammatory Response in BV2 Microglia Cells by Activating LXRα.
Liu, Bin; He, Zhaoqi; Wang, Jingjing; Xin, Zhuoyuan; Wang, Jiaxin; Li, Fan; Fu, Yunhe.
Afiliação
  • Liu B; Cardiovascular Disease Center, First Hospital of Jilin University, Changchun, China.
  • He Z; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, China.
  • Wang J; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, China.
  • Xin Z; Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, China.
  • Wang J; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, China.
  • Li F; Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, China.
  • Fu Y; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, China.
Front Pharmacol ; 9: 278, 2018.
Article em En | MEDLINE | ID: mdl-29670526
Neuroinflammation plays a critical role in the development of neurodegenerative diseases. Taraxasterol, a pentacyclic-triterpene isolated from Taraxacum officinale, has been reported to have anti-inflammatory effect. The aim of this study was to investigate the anti-inflammatory effects and mechanism of taraxasterol in LPS-stimulated BV2 microglia cells. BV2 microglia cells were treated with taraxasterol 12 h before LPS stimulation. The effects of taraxasterol on LPS-induced TNF-α and IL-1ß production were detected by ELISA. The effects of taraxasterol on LXRα, ABCA1, TLR4, and NF-κB expression were detected by western blot analysis. The results showed that taraxasterol dose-dependently inhibited LPS-induced TNF-α and IL-1ß production and NF-κB activation. Taraxasterol also disrupted the formation of lipid rafts and inhibited translocation of TLR4 into lipid rafts. Furthermore, taraxasterol was found to activate LXRα-ABCA1 signaling pathway which induces cholesterol efflux from cells. In addition, our results showed that the anti-inflammatory effect of taraxasterol was attenuated by transfection with LXRα siRNA. In conclusion, these results suggested that taraxasterol inhibits LPS-induced inflammatory response in BV2 microglia cells by activating LXRα-ABCA1 signaling pathway.
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2018 Tipo de documento: Article