Enabling multiplexed testing of pooled donor cells through whole-genome sequencing.
Genome Med
; 10(1): 31, 2018 04 19.
Article
em En
| MEDLINE
| ID: mdl-29673390
ABSTRACT
We describe a method that enables the multiplex screening of a pool of many different donor cell lines. Our method accurately predicts each donor proportion from the pool without requiring the use of unique DNA barcodes as markers of donor identity. Instead, we take advantage of common single nucleotide polymorphisms, whole-genome sequencing, and an algorithm to calculate the proportions from the sequencing data. By testing using simulated and real data, we showed that our method robustly predicts the individual proportions from a mixed-pool of numerous donors, thus enabling the multiplexed testing of diverse donor cells en masse.More information is available at https//pgpresearch.med.harvard.edu/poolseq/.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doadores de Tecidos
/
Linfócitos B
/
Sequenciamento de Nucleotídeos em Larga Escala
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Sequenciamento Completo do Genoma
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article