[Application of DNA-based electrochemical biosensor in rapid detection of Escherichia coli exist in licorice decoction].
Zhongguo Zhong Yao Za Zhi
; 43(6): 1209-1214, 2018 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-29676130
ABSTRACT
A new method for detection of Escherichia coli exist in licorice decoction was developed by using DNA-based electrochemical biosensor. The thiolated capture probe was immobilized on a gold electrode at first. Then the aptamer for Escherichia coli was combined with the capture probe by hybridization. Due to the stronger interaction between the aptamer and the E. coli, the aptamer can dissociate from the capture probe in the presence of E. coli in licorice decoction. The biotinylated detection probe was hybridized with the single-strand capture probe. As a result, the electrochemical response to Escherichia coli can be measured by using differential pulse voltammetric in the presence of α-naphthyl phosphate. The plot of peak current vs. the logarithm of concentration in the range from 2.7×10² to 2.7×108 CFU·mL⻹ displayed a linear relationship with a detection limit of 50 CFU·mL⻹. The relative standard deviation of 3 successive scans was 2.5%ï¼2.1%ï¼4.6% for 2×10²ï¼2×104ï¼2×106ï¼6 CFU·mL⻹ E. coli, respectively. The proposed procedure showed better specificity to E. coli in comparison to Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. In the detection of the real extractum glycyrrhizae, the results between the proposed strategy and the GB assay showed high degree of agreement, demonstrating the designed biosensor could be utilized as a powerful tool for microbial examination for traditional Chinese medicine.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Extratos Vegetais
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Técnicas Biossensoriais
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Escherichia coli
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Glycyrrhiza
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article