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An optimized method to measure human FOXP3+ regulatory T cells from multiple tissue types using mass cytometry.
Dawson, Nicholas A J; Lam, Avery J; Cook, Laura; Hoeppli, Romy E; Broady, Raewyn; Pesenacker, Anne M; Levings, Megan K.
Afiliação
  • Dawson NAJ; Department of Medicine, University of British Columbia Vancouver, BC, Canada.
  • Lam AJ; BC Children's Hospital Research Institute, Vancouver, BC, Canada.
  • Cook L; BC Children's Hospital Research Institute, Vancouver, BC, Canada.
  • Hoeppli RE; Department of Surgery, University of British Columbia, Vancouver, BC, Canada.
  • Broady R; Department of Medicine, University of British Columbia Vancouver, BC, Canada.
  • Pesenacker AM; BC Children's Hospital Research Institute, Vancouver, BC, Canada.
  • Levings MK; BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Eur J Immunol ; 48(8): 1415-1419, 2018 08.
Article em En | MEDLINE | ID: mdl-29676458
We optimized a method to detect FOXP3 by mass cytometry and compared the resulting data to conventional flow cytometry. We also demonstrated the utility of the protocol to profile antigen-specific Tregs from whole blood, or Tregs from tissues such as cord blood, thymus and synovial fluid.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos T Reguladores / Contagem de Linfócito CD4 / Fatores de Transcrição Forkhead / Citometria de Fluxo Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos T Reguladores / Contagem de Linfócito CD4 / Fatores de Transcrição Forkhead / Citometria de Fluxo Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article