Engineering l-asparaginase for spontaneous formation of calcium phosphate bioinspired microreactors.
Phys Chem Chem Phys
; 20(18): 12719-12726, 2018 May 09.
Article
em En
| MEDLINE
| ID: mdl-29697113
ABSTRACT
Active bioinspired materials are appealing biotechnological targets, and their study is gaining momentum. These materials, which comprise of an inorganic matrix and one or more biomolecules, are extremely variable and therefore may result difficult to characterize in their intimate structure. In this work we have prepared a hydroxyapatite-l-asparaginase composite, with the perspective of using it in acute leukemia treatment. We demonstrate that the use of electron microscopy and powder X-ray diffraction, combined with the atomic-resolution information coming from solid-state NMR, allows us to understand the topology of the material and how the different components interplay to obtain an active composite.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Asparaginase
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Materiais Biocompatíveis
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Engenharia de Proteínas
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Durapatita
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Proteínas de Escherichia coli
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article