Reactive oxygen species dynamics in roots of salt sensitive and salt tolerant cultivars of rice.
Anal Biochem
; 550: 99-108, 2018 06 01.
Article
em En
| MEDLINE
| ID: mdl-29704477
ABSTRACT
Salinity stress is one of the major constraints for growth and survival of plants that affects rice productivity worldwide. Hence, in the present study, roots of two contrasting salinity sensitive cultivars, IR64 (IR64, salt sensitive) and Luna Suvarna (LS, salt tolerant) were compared with regard to the levels of reactive oxygen species (ROS) to derive clues for their differential salt stress adaptation mechanisms. In our investigation, the tolerant cultivar exhibited longer primary roots, more lateral roots, higher root number leading to increased root biomass, with respect to IR64. It was observed that LS roots maintained higher level of H2O2 in comparison to IR64. The activities of various enzymes involved in enzymatic antioxidant defense mechanism (SOD, CAT, GPX, DHAR and MDHAR) were found to be greater in LS roots. Further, the higher transcript level accumulation of genes encoding ROS generating (RbohA, RbohD and RbohE) and scavenging enzymes (Fe-SOD, Chloroplastic Cu/Zn-SOD, CAT and DHAR) were noticed in the roots of tolerant cultivar, LS. Moreover, the content of other stress markers such as total protein and proline were also elevated in LS roots. While, the expression of proline biosynthesis gene (P5CS) and proline catabolism gene (PDH) was observed to be lower in LS.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
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Proteínas de Plantas
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Oryza
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Regulação Enzimológica da Expressão Gênica
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Raízes de Plantas
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Regulação da Expressão Gênica de Plantas
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Plantas Tolerantes a Sal
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Peróxido de Hidrogênio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article