Receptor-mediated PLGA nanoparticles for glioblastoma multiforme treatment.
Int J Pharm
; 545(1-2): 84-92, 2018 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-29715532
ABSTRACT
Glioblastoma multiforme is the most lethal type of brain tumor and the established therapy only extends patients survival to approximately one year. Its first-line treatment is based on of chemotherapy with the alkylating agent temozolomide (TMZ). As many other chemotherapeutic drugs, TMZ presents several limitations as high toxicity and low bioavailability. The delivery of TMZ using poly(lactic-co-glycolic acid) nanoparticles is proposed in this work. Stable nanoparticles functionalized with a OX26 type monoclonal antibody for transferrin receptor were developed, targeting the glioblastoma tumor cells, since these cells are known for overexpressing this receptor. The release profile of TMZ from the nanoparticles was studied mimicking physiological conditions, and targeted cellular internalization was also investigated. Two glioblastoma cell lines - U215 and U87 - were used to evaluate the in vitro cytotoxicity of the drug, showing that the prepared nanocarriers enhance the anticancer activity of TMZ. The functionalization with the monoclonal antibody for transferrin receptor proved to be advantageous in enhancing the cellular internalization in glioblastoma cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Poliglicólico
/
Receptores da Transferrina
/
Neoplasias Encefálicas
/
Portadores de Fármacos
/
Glioblastoma
/
Antineoplásicos Alquilantes
/
Ácido Láctico
/
Dacarbazina
/
Nanopartículas
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article