OMNY-A tOMography Nano crYo stage.
Rev Sci Instrum
; 89(4): 043706, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-29716370
ABSTRACT
For many scientific questions gaining three-dimensional insight into a specimen can provide valuable information. We here present an instrument called "tOMography Nano crYo (OMNY)," dedicated to high resolution 3D scanning x-ray microscopy at cryogenic conditions via hard X-ray ptychography. Ptychography is a lens-less imaging method requiring accurate sample positioning. In OMNY, this in achieved via dedicated laser interferometry and closed-loop position control reaching sub-10 nm positioning accuracy. Cryogenic sample conditions are maintained via conductive cooling. 90 K can be reached when using liquid nitrogen as coolant, and 10 K is possible with liquid helium. A cryogenic sample-change mechanism permits measurements of cryogenically fixed specimens. We compare images obtained with OMNY with older measurements performed using a nitrogen gas cryo-jet of stained, epoxy-embedded retina tissue and of frozen-hydrated Chlamydomonas cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tomografia por Raios X
/
Imageamento Tridimensional
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Microscopia
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article