Sex Determination in Ceratopteris richardii Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte.
G3 (Bethesda)
; 8(7): 2205-2214, 2018 07 02.
Article
em En
| MEDLINE
| ID: mdl-29720393
ABSTRACT
The fern Ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to de novo assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This C. richardii gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants.
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MEDLINE
Assunto principal:
Genes de Plantas
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Processos de Determinação Sexual
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Gleiquênias
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Epigênese Genética
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Células Germinativas Vegetais
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Transcriptoma
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article