Growing Murine Bone Marrow-Derived Macrophages.
Methods Mol Biol
; 1784: 29-33, 2018.
Article
em En
| MEDLINE
| ID: mdl-29761385
ABSTRACT
Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains. They are therefore widely used as prototypical macrophages for in vitro studies. In this chapter, we present the method for obtaining BMDMs and freezing them.
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Técnicas de Cultura de Células
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Macrófagos
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article