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The First Recombinant Viper Three-Finger Toxins: Inhibition of Muscle and Neuronal Nicotinic Acetylcholine Receptors.
Makarova, Ya V; Kryukova, E V; Shelukhina, I V; Lebedev, D S; Andreeva, T V; Ryazantsev, D Yu; Balandin, S V; Ovchinnikova, T V; Tsetlin, V I; Utkin, Yu N.
Afiliação
  • Makarova YV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. yanson1@yandex.ru.
  • Kryukova EV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Shelukhina IV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Lebedev DS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Andreeva TV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Ryazantsev DY; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Balandin SV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Ovchinnikova TV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
  • Tsetlin VI; Sechenov First Moscow State Medical University, Ministry of Healthcare of the Russian Federation, Moscow, 119992, Russia.
  • Utkin YN; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Dokl Biochem Biophys ; 479(1): 127-130, 2018 Mar.
Article em En | MEDLINE | ID: mdl-29779115
ABSTRACT
Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii, respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. It was shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors of neuronal and muscle type.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Biológicas / Proteínas Recombinantes / Receptores Nicotínicos / Viperidae / Músculos / Neurônios Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Biológicas / Proteínas Recombinantes / Receptores Nicotínicos / Viperidae / Músculos / Neurônios Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article