Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.
Blood
; 132(5): e1-e12, 2018 08 02.
Article
em En
| MEDLINE
| ID: mdl-29784642
ABSTRACT
Platelet integrity and function critically depend on lipid composition. However, the lipid inventory in platelets was hitherto not quantified. Here, we examined the lipidome of murine platelets using lipid-category tailored protocols on a quantitative lipidomics platform. We could show that the platelet lipidome comprises almost 400 lipid species and covers a concentration range of 7 orders of magnitude. A systematic comparison of the lipidomics network in resting and activated murine platelets, validated in human platelets, revealed that <20% of the platelet lipidome is changed upon activation, involving mainly lipids containing arachidonic acid. Sphingomyelin phosphodiesterase-1 (Smpd1) deficiency resulted in a very specific modulation of the platelet lipidome with an order of magnitude upregulation of lysosphingomyelin (SPC), and subsequent modification of platelet activation and thrombus formation. In conclusion, this first comprehensive quantitative lipidomic analysis of platelets sheds light on novel mechanisms important for platelet function, and has therefore the potential to open novel diagnostic and therapeutic opportunities.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosforilcolina
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Esfingomielina Fosfodiesterase
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Esfingosina
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Trombose
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Plaquetas
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Lipídeos
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article