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Adult rat myelin enhances axonal outgrowth from neural stem cells.
Poplawski, Gunnar H D; Lie, Richard; Hunt, Matt; Kumamaru, Hiromi; Kawaguchi, Riki; Lu, Paul; Schäfer, Michael K E; Woodruff, Grace; Robinson, Jacob; Canete, Philip; Dulin, Jennifer N; Geoffroy, Cedric G; Menzel, Lutz; Zheng, Binhai; Coppola, Giovanni; Tuszynski, Mark H.
Afiliação
  • Poplawski GHD; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Lie R; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Hunt M; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Kumamaru H; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Kawaguchi R; Departments of Psychiatry and Neurology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Lu P; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Schäfer MKE; Veterans Administration Medical Center, San Diego, CA 92161, USA.
  • Woodruff G; Department of Anesthesiology and Focus Program Translational Neurosciences, Medical Center of the Johannes Gutenberg-University, Mainz, Germany.
  • Robinson J; Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
  • Canete P; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Dulin JN; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Geoffroy CG; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Menzel L; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Zheng B; Department of Anesthesiology and Focus Program Translational Neurosciences, Medical Center of the Johannes Gutenberg-University, Mainz, Germany.
  • Coppola G; Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Tuszynski MH; Departments of Psychiatry and Neurology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Sci Transl Med ; 10(442)2018 05 23.
Article em En | MEDLINE | ID: mdl-29794059
ABSTRACT
Axon regeneration after spinal cord injury (SCI) is attenuated by growth inhibitory molecules associated with myelin. We report that rat myelin stimulated the growth of axons emerging from rat neural progenitor cells (NPCs) transplanted into sites of SCI in adult rat recipients. When plated on a myelin substrate, neurite outgrowth from rat NPCs and from human induced pluripotent stem cell (iPSC)-derived neural stem cells (NSCs) was enhanced threefold. In vivo, rat NPCs and human iPSC-derived NSCs extended greater numbers of axons through adult central nervous system white matter than through gray matter and preferentially associated with rat host myelin. Mechanistic investigations excluded Nogo receptor signaling as a mediator of stem cell-derived axon growth in response to myelin. Transcriptomic screens of rodent NPCs identified the cell adhesion molecule neuronal growth regulator 1 (Negr1) as one mediator of permissive axon-myelin interactions. The stimulatory effect of myelin-associated proteins on rodent NPCs was developmentally regulated and involved direct activation of the extracellular signal-regulated kinase (ERK). The stimulatory effects of myelin on NPC/NSC axon outgrowth should be investigated further and could potentially be exploited for neural repair after SCI.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Axônios / Envelhecimento / Células-Tronco Neurais / Crescimento Neuronal / Bainha de Mielina Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Axônios / Envelhecimento / Células-Tronco Neurais / Crescimento Neuronal / Bainha de Mielina Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article