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Flow cytometry as a new complementary tool to study Coxiella burnetii in cell cultures.
Rebbig, Annica; Matthiesen, Svea; Lührmann, Anja; Knittler, Michael R.
Afiliação
  • Rebbig A; Friedrich-Loeffler-Institut, Institute of Immunology, Federal Research Institute of Animal Health, Südufer 10, D-17493 Greifswald, Isle of Riems, Germany.
  • Matthiesen S; Friedrich-Loeffler-Institut, Institute of Immunology, Federal Research Institute of Animal Health, Südufer 10, D-17493 Greifswald, Isle of Riems, Germany.
  • Lührmann A; Institute for Clinical Microbiology, Immunology and Hygiene of the University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Wasserturmstraße 3/5, D-91054 Erlangen, Germany.
  • Knittler MR; Friedrich-Loeffler-Institut, Institute of Immunology, Federal Research Institute of Animal Health, Südufer 10, D-17493 Greifswald, Isle of Riems, Germany. Electronic address: michael.knittler@fli.de.
J Microbiol Methods ; 151: 39-43, 2018 08.
Article em En | MEDLINE | ID: mdl-29857017
ABSTRACT
Flow cytometry enables the analysis of cells by labeling them with fluorescent probes. We describe a novel flow cytometric approach permitting reliable analysis of Coxiella (C.) burnetii-infected cells. The method quantifies infection-forming units (IFUs) in a dose-dependent manner and allows for the specific detection of infection/replication-competent coxiella in cell cultures.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coxiella burnetii / Citometria de Fluxo Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coxiella burnetii / Citometria de Fluxo Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article