A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium.
Microbes Environ
; 33(2): 227-229, 2018 Jul 04.
Article
em En
| MEDLINE
| ID: mdl-29863058
ABSTRACT
This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.
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Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
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Complexo IV da Cadeia de Transporte de Elétrons
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Estramenópilas
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Microalgas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article