Simple and robust LC-MS/MS analysis method for therapeutic drug monitoring of micafungin.
Bioanalysis
; 10(11): 877-886, 2018 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-29863416
ABSTRACT
AIM:
To develop a simple and robust LC-MS/MS method to quantify concentrations of micafungin in human plasma for pharmacokinetic studies and therapeutic drug monitoring.METHODS:
Sample preparation involved protein precipitation with acetonitrilemethanol (8317% v/v) and [13C6]-micafungin as internal standard. A rapid and selective method for micafungin was validated across a range of 0.200-10.0 mg/l.RESULTS:
The calculated accuracy for the eight-point calibration ranged from 0.7 to 5.3%. Within-run precision ranged from 0.8 to 5.9%, between-run precision ranged from 0.7 to 3.1%, and overall precision ranged from 1.3 to 6.6%.CONCLUSION:
A simple and robust LC-MS/MS method for analyzing micafungin in human plasma has been validated and was utilized for quantification of micafungin.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Monitoramento de Medicamentos
/
Espectrometria de Massas em Tandem
/
Equinocandinas
/
Lipopeptídeos
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article