Simple and robust LC-MS/MS analysis method for therapeutic drug monitoring of micafungin.

ABSTRACT

AIM: To develop a simple and robust LC-MS/MS method to quantify concentrations of micafungin in human plasma for pharmacokinetic studies and therapeutic drug monitoring. METHODS: Sample preparation involved protein precipitation with acetonitrilemethanol (8317% v/v) and [13C6]-micafungin as internal standard. A rapid and selective method for micafungin was validated across a range of 0.200-10.0 mg/l. RESULTS: The calculated accuracy for the eight-point calibration ranged from 0.7 to 5.3%. Within-run precision ranged from 0.8 to 5.9%, between-run precision ranged from 0.7 to 3.1%, and overall precision ranged from 1.3 to 6.6%. CONCLUSION: A simple and robust LC-MS/MS method for analyzing micafungin in human plasma has been validated and was utilized for quantification of micafungin.