An insertion within a variably spliced Drosophila tropomyosin gene blocks accumulation of only one encoded isoform.
Cell
; 41(1): 57-66, 1985 May.
Article
em En
| MEDLINE
| ID: mdl-2986850
ABSTRACT
We have characterized an aberrant allele of a variably spliced Drosophila tropomyosin gene. The allele was recovered from the flightless Ifm(3)3 strain, which has been shown to have structurally and functionally abnormal indirect flight muscles. The transcribed portion of the mutant gene is interrupted by an 8,8 kb insertion of middle repetitive DNA having a structure typical of copia-like Drosophila mobile elements. The insertion is positioned so as to interrupt an exon sequence in one splicing mode and, simultaneously, an intron in the alternate mode. As a consequence of the insertion the allele fails to direct synthesis of the flight muscle-specific tropomyosin isoform, but remains capable of specifying a second isoform, which accumulates in nonfibrillar Drosophila muscles.
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Base de dados:
MEDLINE
Assunto principal:
Tropomiosina
/
Elementos de DNA Transponíveis
/
Splicing de RNA
/
Drosophila
Limite:
Animals
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article