Use of bioreactors for culturing human retinal organoids improves photoreceptor yields.
Stem Cell Res Ther
; 9(1): 156, 2018 06 13.
Article
em En
| MEDLINE
| ID: mdl-29895313
ABSTRACT
BACKGROUND:
The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue is a lengthy process, often taking over 6 months of cell culture, and current approaches do not always generate large quantities of the major retinal cell types required.METHODS:
We adapted our previously described differentiation protocol to investigate the use of stirred-tank bioreactors. We used immunohistochemistry, flow cytometry and electron microscopy to characterise retinal organoids grown in standard and bioreactor culture conditions.RESULTS:
Our analysis revealed that the use of bioreactors results in improved laminar stratification as well as an increase in the yield of photoreceptor cells bearing cilia and nascent outer-segment-like structures.CONCLUSIONS:
Bioreactors represent a promising platform for scaling up the manufacture of retinal cells for use in disease modelling, drug screening and cell transplantation studies.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Fotorreceptoras
/
Retina
/
Organoides
/
Reatores Biológicos
/
Células-Tronco Pluripotentes
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article