[Based on in vivo fluorescence imaging technology, to extablish a fluorescence modification method for amphipathic block polymers].

ABSTRACT

Rhodamine B (Rh B) was used to decorate an amphipathic block polymers (ß-CD-[P(AA- co-MMA)-b-PVP](4)) in this study. First, after activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, rhodamine B was marked with hydroxyethyl methacrylate (HEMA) through ester exchange reaction. Second, the labeled amphipathic block polymers (ß-CD-[P(AA-(HEMA-RhB)-MMA)-b-PVP](4)) were synthesized after polymerization reaction of double bones between Rh B-HEMA and other reactants. Finally, the structure of product was measured by FT-IR spectra and fluorospectro photometer (FLUORO). The critical micelle concentration of Rh B-labeled and unlabeled amphipathic block polymers were 4.96×10(-3), 5.09×10(-3)mg·L(-1), respectively, indicating no change of their micellization behavior. In vivo tissue distribution and whole- body fluorescent imaging were studied by vinpocetine (VP)-loaded polymeric micelles which were prepared through a solvent evaporation method. Compared to the result of in vivo tissue distribution and whole-body fluorescence imaging, a similar bio-distribution behavior of VP-loaded polymeric micelles was found. Those proved the successful fluorescence modification with a labeling yield of 4.13%. With in vivo fluorescence imaging technology, we established a fluorescence method for modification of amphipathic block polymers.