Simvastatin and bone marrow-derived mesenchymal stem cells (BMSCs) affects serum IgE and lung cytokines levels in sensitized mice.
Cytokine
; 113: 83-88, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-29914792
ABSTRACT
INTRODUCTION:
The effects of bone marrow-derived mesenchymal stem cells (BMSCs) and simvastatin combination therapy on serum total and specific IgE levels and lung IL-13 and TGF-ß levels in sensitized mouse were examined. MATERIAL ANDMETHODS:
Control (nâ¯=â¯5), Sensitized (S), (nâ¯=â¯5), Sâ¯+â¯BMSC (nâ¯=â¯6), Sâ¯+â¯simvastatin (nâ¯=â¯6) and Sâ¯+â¯BMSCâ¯+â¯simvastatin (nâ¯=â¯4) groups of BALB/c mice were studied. Mice were sensitized by ovalbumin. Sensitized mice were treated with a single intravenous injection of BMSCs (1â¯×â¯106) or daily intraperitoneal injection of simvastatin (40â¯mg/kg) or both BMSCs and simvastatin on the last week of challenge. Serum total and ovalbumin specific IgE levels as well as IL-13 and TGF-ß levels in broncho-alveolar lavage (BAL) fluid were evaluated.RESULTS:
Serum total and specific IgE levels as well as lung IL-13 and TGF-ß levels were significantly increased in S group compared to control group (Pâ¯<â¯0.001 for all cases). Treatment with BMSCs, simvastatin and their combination significantly decreased serum total and specific IgE levels (Pâ¯<â¯0.05 to Pâ¯<â¯0.01). However, IL-13 and TGF-ß levels were significantly decreased by BMSCs and BMSCâ¯+â¯simvastatin combination therapy (Pâ¯<â¯0.05 for all cases). The effect of simvastatin and BMSCs combination therapy on serum specific IgE levels as well as lung IL-13 and TGF-ß levels were significantly higher than the effect of BMSCs and simvastatin alone (Pâ¯<â¯0.001 for IL-13 and Pâ¯<â¯0.01 for other cases).CONCLUSIONS:
Simvastatin and BMSCs combination therapy affects serum IgE as well as lung IL-13 and TGFß levels more than BMSC therapy and simvastatin therapy alone which may be due to increased BMSCs migration into the lung tissue.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Imunoglobulina E
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Sinvastatina
/
Células-Tronco Mesenquimais
/
Pulmão
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article