Large-scale production of polyoma middle T antigen by using genetically engineered tumors.
Mol Cell Biol
; 5(7): 1795-9, 1985 Jul.
Article
em En
| MEDLINE
| ID: mdl-2991752
ABSTRACT
A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polyomavirus
/
Antígenos Virais de Tumores
Limite:
Animals
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article