[Effects of centellaasiatica granule on the expression of Smad 2/3, Smad 7 and collagen â
£ in the mesangial cells stably expressed TGF-ß1].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 34(2): 122-125, 2018 Feb 08.
Article
em Zh
| MEDLINE
| ID: mdl-29926674
ABSTRACT
OBJECTIVES:
Stably expressed transforming growth factor -beta 1(TGF-ß1)MCs were obtained and the effects of centellaasiatica (CA) granule on the expressions of Smad 2/3, Smad 7 and collagen â £ and the level of Smad 2/3 phosphorylation were observed.METHODS:
Lipofectin method was used to transfect TGF-ß1 vector into MC, and the stably expressed TGF-ß1 cell lines were selected by G418. The cells were divided into three groups. Control groupnormal MC + RPMI 1640 + 10% normal rat serum; TGF-ß1 groupstably expressed TGF-ß1 MC + RPMI 1640 + 10% normal rat serum; CA groupstably expressed TGF-ß1 MC + RPMI 1640 + 10% rat serum containing high CA. The experiments were repeated for five times. The contents of TGF-ß1 and collagen â £ in the culture medium were detected with ELISA, the expressions of mRNA and protein of TGF-ß1, Smad 2/3, Smad 7 and the level of Smad 2/3 phosphorylation were detected by using real time quantitative polymerase chain reaction and Western blot.RESULTS:
The contents of TGF-ß1 and collagen â £ in the culture medium of stably-expressed TGF-ß1 MC were increased significantly, and the CA could reverse the effects of TGF-ß1. The expressions of mRNA and protein of TGF-ß1, Smad 2/3 and the level of Smad 2/3 phosphorylation were increased significantly in TGF-ß1 transfected MC, and CA could dramatically reduce the expressions of mRNA and protein of TGF-ß1, Smad 2/3 and the level of Smad 2/3 phosphorylation. The high expression of TGF-ß1 decreased the expression of Smad 7 mRNA and protein, and the CA could antagonize the effect of mRNA expression.CONCLUSIONS:
The MCs stably-expressed TGF-ß1 can activate the TGF-ß1/Smad signal pathway and increase the expression of collagen â £. CA can decrease the occurrence of diabetic nephropathy(DN) by reducing the production of collagen â £ through inhibiting the TGF-ß1/Smad signal pathway.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Centella
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Colágeno Tipo IV
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Células Mesangiais
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Proteínas Smad
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Fator de Crescimento Transformador beta1
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article