Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera.
Vet Microbiol
; 10(4): 325-33, 1985 Jun.
Article
em En
| MEDLINE
| ID: mdl-2994278
ABSTRACT
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was established for the detection of antibodies to bovine viral diarrhea virus (BVDV) in bovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified BVDV was used as test antigen at an optimal amount of 1 microgram/well, whereas the optimal concentration of conjugate was at 1/2000 dilution. The standardized test encountered no non-specific reaction with test sera at a starting dilution of 1/10. A total of 50 bovine serum samples was assayed for the presence of antibodies against BVDV by ELISA and serum neutralization test (SNT). A positive correlation between the 2 tests was found. However, ELISA could be as much as 500-fold more sensitive than SNT in detecting low levels of BVDV antibodies.
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Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Ensaio de Imunoadsorção Enzimática
/
Técnicas Imunoenzimáticas
/
Pestivirus
/
Vírus da Diarreia Viral Bovina
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article