Quantitative localization of [3H]TCP binding in rat brain by light microscopy autoradiography.
Brain Res
; 344(1): 142-5, 1985 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-2994834
The anatomical localization of phencyclidine (PCP)/sigma-opiate receptors in rat brain was determined by quantitative light microscopy autoradiography using the new ligand N-(1-[2-thienyl]cyclohexyl) [3H]piperidine ([3H]TCP). TCP is a potent analog of PCP which possesses a higher affinity for PCP/sigma-opiate receptor than does PCP itself. The highest level of [3H]TCP binding was detected in the hippocampus. Intermediate levels were found in frontal cortex, striatum, amygdala and cerebellum. Specific [3H]TCP binding was undetectable in anterior commissure and corpus callosum. The distribution pattern of [3H]TCP binding sites is similar to the pattern obtained with [3H]PCP but more sharply defined. On the basis of its greater potency and specificity, [3H]TCP may prove superior to [3H]PCP as a molecular probe for the study of brain sigma opiate/phencyclidine receptors.
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Base de dados:
MEDLINE
Assunto principal:
Fenciclidina
/
Encéfalo
/
Receptores Opioides
Limite:
Animals
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article