Discovery of a dual protease mechanism that promotes DNA damage checkpoint recovery.
PLoS Genet
; 14(7): e1007512, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29979679
ABSTRACT
The DNA damage response is a signaling pathway found throughout biology. In many bacteria the DNA damage checkpoint is enforced by inducing expression of a small, membrane bound inhibitor that delays cell division providing time to repair damaged chromosomes. How cells promote checkpoint recovery after sensing successful repair is unknown. By using a high-throughput, forward genetic screen, we identified two unrelated proteases, YlbL and CtpA, that promote DNA damage checkpoint recovery in Bacillus subtilis. Deletion of both proteases leads to accumulation of the checkpoint protein YneA. We show that DNA damage sensitivity and increased cell elongation in protease mutants depends on yneA. Further, expression of YneA in protease mutants was sufficient to inhibit cell proliferation. Finally, we show that both proteases interact with YneA and that one of the two proteases, CtpA, directly cleaves YneA in vitro. With these results, we report the mechanism for DNA damage checkpoint recovery in bacteria that use membrane bound cell division inhibitors.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
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Bacillus subtilis
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Proteínas de Bactérias
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Dano ao DNA
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Reparo do DNA
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article