Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.
Nat Methods
; 15(8): 601-604, 2018 08.
Article
em En
| MEDLINE
| ID: mdl-29988095
ABSTRACT
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Luminescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article