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Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.
Pennacchietti, Francesca; Serebrovskaya, Ekaterina O; Faro, Aline R; Shemyakina, Irina I; Bozhanova, Nina G; Kotlobay, Alexey A; Gurskaya, Nadya G; Bodén, Andreas; Dreier, Jes; Chudakov, Dmitry M; Lukyanov, Konstantin A; Verkhusha, Vladislav V; Mishin, Alexander S; Testa, Ilaria.
Afiliação
  • Pennacchietti F; Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Serebrovskaya EO; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Faro AR; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
  • Shemyakina II; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Bozhanova NG; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Kotlobay AA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Gurskaya NG; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Bodén A; Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Dreier J; Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Chudakov DM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Lukyanov KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
  • Verkhusha VV; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
  • Mishin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation. mishin@ibch.ru.
  • Testa I; Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. ilaria.testa@scilifelab.se.
Nat Methods ; 15(8): 601-604, 2018 08.
Article em En | MEDLINE | ID: mdl-29988095
ABSTRACT
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Luminescentes Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Luminescentes Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article