Proteomic profile changes associated with diminished expression of T-cell intracellular antigens reveal a hormesis response.
Biochem Biophys Res Commun
; 503(4): 2569-2575, 2018 09 18.
Article
em En
| MEDLINE
| ID: mdl-30017198
ABSTRACT
T-cell intracellular antigen (TIA) proteins function as regulators of cell homeostasis by controlling global gene expression in response to dynamic regulatory changes and environmental stress. Here, we used two-dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry (MALDI-TOF/TOF) to identify protein changes associated with the down-regulated expression of TIA proteins. We detected 30 differentially expressed proteins (DEPs), 24 of which were identified, and some of these DEPs were validated by western blotting. In silico analysis showed that DEPs were associated with metabolic processes, detoxification and proteostasis. We mapped the DEPs to the available biological pathways and networks, which included the metabolism of small molecules such as sugars, lipids, amino acids, and nucleotides. Our findings support previous studies and suggest that low expression of TIA proteins might act as a potential adaptive switch to link gene expression reprogramming to a proliferative phenotype mediated by a hormesis phenomenon.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
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Perfilação da Expressão Gênica
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Proteômica
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Hormese
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Antígenos
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article