Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene.
J Microbiol Methods
; 152: 7-9, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30017848
ABSTRACT
We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker.
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Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
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Proteínas Fúngicas
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Biomarcadores
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Marcadores Genéticos
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Pleurotus
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article