MiR-550a-3p promotes non-small cell lung cancer cell proliferation and metastasis through down-regulating TIMP2.
Eur Rev Med Pharmacol Sci
; 22(13): 4156-4165, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-30024604
OBJECTIVE: MicroRNAs (miRNAs) have been identified to play a crucial regulatory role in the development and progression of malignant tumors, including lung cancer. However, the function of miR-550a-3p on the progression of non-small cell lung cancer (NSCLC) remains poorly understood. PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction was employed to estimate the expression level of miR-550a-3p in NSCLC tissue and cell samples. Cell proliferation was measured by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and colony formation assays. Transwell assay was recruited to demonstrate the abilities of cell invasion and migration. Luciferase analysis and Western-blot assay were performed to elucidate the underlying mechanism of miR-550a-3p in NSCLC. RESULTS: The level of miR-550a-3p expressed in NSCLC tissues was significantly higher than that in para-tumor control tissues. Over-expression of miR-550a-3p significantly promoted proliferation, invasion, and migration of A549 cells while knockdown of miR-550a-3p inhibited growth and metastasis of H460 cells. TIMP2 was verified as a direct target of miR-550a-3p in NSCLC. Restoration of TIMP2 rescued the influence of miR-550a-3p over-expression. CONCLUSIONS: We demonstrated that miR-550a-3p regulated the progression of NSCLC cells through TIMP2. Thus, miR-550a-3p axis could serve as a potential therapeutic target for NSCLC treatment.
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma Pulmonar de Células não Pequenas
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MicroRNAs
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Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article