Morphological changes induced in erythrocyte by amyloid beta peptide and glucose depletion: A combined atomic force microscopy and biochemical study.
Biochim Biophys Acta Biomembr
; 1861(1): 236-244, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30040926
ABSTRACT
Circulating red blood cells (RBCs) undergo aging, a fundamental physiological phenomenon that regulates their turnover. We show that treatment with beta amyloid peptide 1-42 (Aß) accelerates the occurrence of morphological and biochemical aging markers in human RBCs and influences the cell metabolism leading to intracellular ATP depletion. The morphological pattern has been monitored using Atomic Force Microscopy (AFM) imaging and measuring the RBCs' plasma membrane roughness employed as a morphological parameter capable to provide information on the structure and integrity of the membrane-skeleton. Results evidence that Aß boosts the development of crenatures and proto-spicules simultaneously to acceleration in the weakening of the cell-cytoskeleton contacts and to the induction of peculiar nanoscale features on the cell membrane. Incubation in the presence of glucose can remove all but the latter Aß-induced effects. Biochemical data demonstrate that contemporaneously to morphological and structural alterations, Aß and glucose depletion trigger a complex signaling pathway involving caspase 3, protein kinase C (PKC) and nitric oxide derived metabolites. As a whole, the collected data revealed that, the damaging path induced by Aß in RBC provide a sequence of morphological and functional intermediates following one another along RBC life span, including (i) an acceleration in the development of shape alteration typically observed along the RBC's aging; (ii) the development of characteristic membrane features on the plasma membrane and (iii) triggering a complex signaling pathway involving caspase 3, PKC and nitric oxide derived metabolites.
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Base de dados:
MEDLINE
Assunto principal:
Precursor de Proteína beta-Amiloide
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Eritrócitos
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Caspase 3
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Glucose
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article