Diversity, frequency and antifungal resistance of Candida species in patients with type 2 diabetes mellitus.
Acta Odontol Scand
; 76(8): 580-586, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30045641
ABSTRACT
OBJECTIVE:
To determine number, species of Candida and Candida resistance to antifungal therapy according to the metabolic control state and the associated salivary changes in patients with type 2 diabetes mellitus (DM2). MATERIALS ANDMETHODS:
Samples of non-stimulated saliva were collected from 52 patients with DM2. Salivary pH was measured and cultured on Sabouraud glucose agar and the values of CFU/ml were calculated. The species were presumptively identified using CHROMagar Candida® plates, and identification was confirmed by polymerase chain reaction (PCR). C. albicans isolates were cultured on SGA tetracycline agar with nystatin and fluconazole diffusion disks to measure susceptibility.RESULTS:
Sixty six percent of the yeasts isolated were Candida albicans, followed by C. glabrata (20.7%). In patients with decompensated DM2, there was an inverse association between HbA1c value and salivary pH. At higher levels of salivary acidification, a greater diversity and quantity of yeasts of the genus Candida were observed. With nystatin, higher inhibition was observed at lower pH.CONCLUSIONS:
The antifungal therapies could be more effective if it consider, qualitative salivary characteristics as pH, that could determine the susceptibility of species of Candida to at least to nystatin, which is the most used antifungal for treatment to oral candidiasis in patients with DM2.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Candida albicans
/
Candidíase Bucal
/
Farmacorresistência Fúngica
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Diabetes Mellitus Tipo 2
/
Antifúngicos
Tipo de estudo:
Prognostic_studies
/
Qualitative_research
Limite:
Adult
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article