Cystine and Theanine Improve Survival after Gut Ischemia-Reperfusion.
Ann Nutr Metab
; 73(2): 131-137, 2018.
Article
em En
| MEDLINE
| ID: mdl-30056446
ABSTRACT
BACKGROUND AND AIM:
Oral administration of cystine and theanine (CT) may modulate antioxidant glutathione (GSH) metabolism, thereby improving outcomes after gut ischemia reperfusion.METHODS:
Experiment 1 Institute of Cancer Research mice (n = 35) were assigned to a Vehicle (n = 11), a CT140 (n = 14), or a CT280 (n = 10) group. The CT140 and 280 groups were given CT at respective dosages of 140 and 280 mg/kg (cystine theanine = 5 2) once daily via gavage for 5 days. All mice underwent 75-min occlusion of the superior mesenteric artery (SMA). Survival after reperfusion was observed. Experiment 2 Mice (n = 67) were pretreated for 5 days (Vehicle n = 24, CT280 n = 20, vehicle/sham n = 23). The Vehicle and CT280 groups underwent 60-min SMA occlusion. Levels of GSH, the oxidized form of GSH, Glutathione-S-S-Glutathione (GSSG), and GSH-related amino acids (cysteine and glutamic acid) in the small intestine, and plasma cytokine (IL-6, IL-1ß, TNFα) levels, were evaluated before (0 h), 3, 6, or 9 h after reperfusion.RESULTS:
Experiment 1 The CT280 group showed significantly better survival than the Vehicle group. Experiment 2 Gut GSSG, cysteine, and glutamic acid levels were higher in the CT280 than in the Vehicle group after reperfusion. Plasma IL-6 and TNFα levels rose more rapidly in the CT280 than in the Vehicle group.CONCLUSION:
Oral administration of CT improves survival after gut I/R, possibly through the modulation of the GSH-redox cycle and cytokine responses.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
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Cistina
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Glutamatos
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article