Roles for ClpXP in regulating the circadian clock in Synechococcus elongatus.
Proc Natl Acad Sci U S A
; 115(33): E7805-E7813, 2018 08 14.
Article
em En
| MEDLINE
| ID: mdl-30061418
ABSTRACT
In cyanobacteria, the KaiABC posttranslational oscillator drives circadian rhythms of gene expression and controls the timing of cell division. The Kai-based oscillator can be reconstituted in vitro, demonstrating that the clock can run without protein synthesis and degradation; however, protein degradation is known to be important for clock function in vivo. Here, we report that strains deficient in the ClpXP1P2 protease have, in addition to known long-period circadian rhythms, an exaggerated ability to synchronize with the external environment (reduced "jetlag") compared with WT strains. Deletion of the ClpX chaperone, but not the protease subunits ClpP1 or ClpP2, results in cell division defects in a manner that is dependent on the expression of a dusk-peaking factor. We propose that chaperone activities of ClpX are required to coordinate clock control of cell division whereas the protease activities of the ClpXP1P2 complex are required to maintain appropriate periodicity of the clock and its synchronization with the external environment.
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MEDLINE
Assunto principal:
Proteínas de Bactérias
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Ritmo Circadiano
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Chaperonas Moleculares
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Synechococcus
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Endopeptidase Clp
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article