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Increasing tolerance of hospital Enterococcus faecium to handwash alcohols.
Pidot, Sacha J; Gao, Wei; Buultjens, Andrew H; Monk, Ian R; Guerillot, Romain; Carter, Glen P; Lee, Jean Y H; Lam, Margaret M C; Grayson, M Lindsay; Ballard, Susan A; Mahony, Andrew A; Grabsch, Elizabeth A; Kotsanas, Despina; Korman, Tony M; Coombs, Geoffrey W; Robinson, J Owen; Gonçalves da Silva, Anders; Seemann, Torsten; Howden, Benjamin P; Johnson, Paul D R; Stinear, Timothy P.
Afiliação
  • Pidot SJ; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Gao W; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Buultjens AH; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Monk IR; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Guerillot R; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Carter GP; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Lee JYH; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Lam MMC; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Grayson ML; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.
  • Ballard SA; Department of Medicine, University of Melbourne, Heidelberg, Victoria 3084, Australia.
  • Mahony AA; Department of Epidemiology and Preventive Medicine, Monash University, Victoria 3800, Australia.
  • Grabsch EA; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
  • Kotsanas D; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.
  • Korman TM; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.
  • Coombs GW; Monash Infectious Diseases, Monash Health, Clayton, Victoria 3168, Australia.
  • Robinson JO; Monash Infectious Diseases, Monash Health, Clayton, Victoria 3168, Australia.
  • Gonçalves da Silva A; Antimicrobial Resistance and Infectious Diseases Research Laboratory, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
  • Seemann T; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Murdoch, Western Australia 6150, Australia.
  • Howden BP; Antimicrobial Resistance and Infectious Diseases Research Laboratory, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
  • Johnson PDR; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Murdoch, Western Australia 6150, Australia.
  • Stinear TP; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.
Sci Transl Med ; 10(452)2018 08 01.
Article em En | MEDLINE | ID: mdl-30068573
ABSTRACT
Alcohol-based disinfectants and particularly hand rubs are a key way to control hospital infections worldwide. Such disinfectants restrict transmission of pathogens, such as multidrug-resistant Staphylococcus aureus and Enterococcus faecium Despite this success, health care infections caused by E. faecium are increasing. We tested alcohol tolerance of 139 hospital isolates of E. faecium obtained between 1997 and 2015 and found that E. faecium isolates after 2010 were 10-fold more tolerant to killing by alcohol than were older isolates. Using a mouse gut colonization model of E. faecium transmission, we showed that alcohol-tolerant E. faecium resisted standard 70% isopropanol surface disinfection, resulting in greater mouse gut colonization compared to alcohol-sensitive E. faecium We next looked for bacterial genomic signatures of adaptation. Alcohol-tolerant E. faecium accumulated mutations in genes involved in carbohydrate uptake and metabolism. Mutagenesis confirmed the roles of these genes in the tolerance of E. faecium to isopropanol. These findings suggest that bacterial adaptation is complicating infection control recommendations, necessitating additional procedures to prevent E. faecium from spreading in hospital settings.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adaptação Fisiológica / Desinfecção das Mãos / Enterococcus faecium / Álcoois Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adaptação Fisiológica / Desinfecção das Mãos / Enterococcus faecium / Álcoois Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article