Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics.
Methods Mol Biol
; 1832: 61-74, 2018.
Article
em En
| MEDLINE
| ID: mdl-30073522
ABSTRACT
DNA replication and subsequent deposition of nucleosomes is critical for the maintenance of the genome and epigenetic inheritance. Experiments using human tissue culture cells harvested at defined stages of the cell cycle can help to elucidate the mechanism of histone deposition and chromatin assembly in detail. Here, we describe a pulsed-SILAC approach to distinguish newly synthesized and deposited histones during S-phase of the cell cycle from parental "old" histones incorporated in previous replications and to decipher posttranslational histone modifications (PTMs).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Ciclo Celular
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Processamento de Proteína Pós-Traducional
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Proteômica
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article