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A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells.
Lin, Haishuang; Du, Qian; Li, Qiang; Wang, Ou; Wang, Zhanqi; Sahu, Neety; Elowsky, Christian; Liu, Kan; Zhang, Chi; Chung, Soonkyu; Duan, Bin; Lei, Yuguo.
Afiliação
  • Lin H; Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Du Q; Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Li Q; Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Wang O; Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Wang Z; Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
  • Sahu N; Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Elowsky C; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Liu K; Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Zhang C; Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
  • Chung S; Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
  • Duan B; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
  • Lei Y; Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 6
Stem Cell Reports ; 11(2): 454-469, 2018 08 14.
Article em En | MEDLINE | ID: mdl-30078557
ABSTRACT
Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 × 107 cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reatores Biológicos / Células-Tronco Pluripotentes / Células Endoteliais / Técnicas de Cultura Celular por Lotes Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reatores Biológicos / Células-Tronco Pluripotentes / Células Endoteliais / Técnicas de Cultura Celular por Lotes Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article