Evaluation of continuous nonaffinity capture chromatography for a recombinant enzyme-optimization for a changing perfusion feedstream and comparison to batch processing.

Thompson, Christopher; Wilson, Kelly; Larkin, Christopher; Lee, Jeong; Wang, William K; Wendeler, Michaela

Thompson, Christopher; Wilson, Kelly; Larkin, Christopher; Lee, Jeong; Wang, William K; Wendeler, Michaela.

Afiliação

Thompson C; Purification Process Sciences, MedImmune LLC, Gaithersburg, MD 20878.
Wilson K; Purification Process Sciences, MedImmune LLC, Gaithersburg, MD 20878.
Larkin C; GTO Analytical, AstraZeneca Pharmaceuticals LP, Gaithersburg, MD, 20878.
Lee J; Cell Culture and Fermentation Sciences, MedImmune LLC, Gaithersburg, MD, 20878.
Wang WK; Purification Process Sciences, MedImmune LLC, Gaithersburg, MD 20878.
Wendeler M; Purification Process Sciences, MedImmune LLC, Gaithersburg, MD 20878.

Biotechnol Prog ; 34(5): 1195-1204, 2018 09.

Article em En | MEDLINE | ID: mdl-30094961

Assuntos

Cromatografia/métodos; Proteínas Recombinantes/metabolismo; Animais; Células CHO; Cromatografia em Gel; Cromatografia por Troca Iônica; Cricetulus; Proteínas Recombinantes/química; Proteínas Recombinantes/isolamento & purificação

Palavras-chave

continuous processing; economic modeling; nonaffinity capture step; perfusion

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Cromatografia Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google