Rapid and efficient induction of functional astrocytes from human pluripotent stem cells.
Nat Methods
; 15(9): 693-696, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30127505
ABSTRACT
The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Astrócitos
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Células-Tronco Pluripotentes
/
Fatores de Transcrição SOX9
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article