Ansamycins with Antiproliferative and Antineuroinflammatory Activity from Moss-Soil-Derived Streptomyces cacaoi subsp. asoensis H2S5.
J Nat Prod
; 81(9): 1984-1991, 2018 09 28.
Article
em En
| MEDLINE
| ID: mdl-30132670
ABSTRACT
Three new 21-membered macrocyclic benzenoid ansamycins, trienomycins J-L (1-3), together with seven known analogues, trienomycins A-G (4-10), were isolated from liquid culture of the moss soil-derived actinomycete Streptomyces cacaoi subsp. asoensis H2S5. The structures of the new compounds were elucidated by extensive NMR spectroscopic analysis and HRESIMS data. The absolute configurations of trienomycins were established by Marfey's method. Antiproliferative assays showed that compound 1 had the greatest activity against HepG2 cells, with an IC50 value of 0.1 µM. The induction of apoptosis of HepG2 cells by 1 was investigated by flow cytometry and evaluation of nuclear morphology. In addition, all of the compounds inhibited nitric oxide production with IC50 values of 0.02 to 8.3 µM, and compounds 1, 4, and 7 were the most potent inhibitors. These findings will facilitate the development of new antineuroinflammatory agents.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
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Streptomyces
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Lactamas Macrocíclicas
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Anti-Inflamatórios
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article