Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors.
RNA
; 24(12): 1721-1737, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30139799
ABSTRACT
Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5' kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Binding is mediated mostly by the two zinc fingers in the C-terminal region of hPcf11. RNA is bound without pronounced sequence-specificity, but extended G-rich sequences appear to be preferred. We discuss the possibility that CF II contributes to the recognition of cleavage/polyadenylation substrates through interaction with G-rich far-downstream sequence elements.
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Base de dados:
MEDLINE
Assunto principal:
Fosfotransferases
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Fatores de Transcrição
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Proteínas Nucleares
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Fatores de Poliadenilação e Clivagem de mRNA
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Complexos Multiproteicos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article