Collagen/Heparin Bi-Affinity Multilayer Modified Collagen Scaffolds for Controlled bFGF Release to Improve Angiogenesis In Vivo.
Macromol Biosci
; 18(11): e1800086, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30160040
ABSTRACT
Basic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long-term controlled release of bFGF by utilizing a collagen/heparin bi-affinity multilayer delivery system (CHBMDS), which is fabricated by the alternate deposition of negatively charged heparin, positively charged collagen, and CBD-bFGF (a collagen-binding domain [CBD] was fused into the native bFGF) via specific or electrostatic interaction. The results show that CHBMDS not only support localized and prolonged release of CBD-bFGF(over 35 days) but also lead to enhanced angiogenesis (higher density and larger diameter (≈70 µm) of newly formed blood vessels in subcutaneous tissue of SD rat after 5 weeks). This system could act as a versatile approach for bFGF delivery and further improve therapeutic efficacy for injured tissues.
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Base de dados:
MEDLINE
Assunto principal:
Heparina
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Fator 2 de Crescimento de Fibroblastos
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Colágeno
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Neovascularização Fisiológica
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Alicerces Teciduais
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article