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Intelligent Image-Activated Cell Sorting.
Nitta, Nao; Sugimura, Takeaki; Isozaki, Akihiro; Mikami, Hideharu; Hiraki, Kei; Sakuma, Shinya; Iino, Takanori; Arai, Fumihito; Endo, Taichiro; Fujiwaki, Yasuhiro; Fukuzawa, Hideya; Hase, Misa; Hayakawa, Takeshi; Hiramatsu, Kotaro; Hoshino, Yu; Inaba, Mary; Ito, Takuro; Karakawa, Hiroshi; Kasai, Yusuke; Koizumi, Kenichi; Lee, SangWook; Lei, Cheng; Li, Ming; Maeno, Takanori; Matsusaka, Satoshi; Murakami, Daichi; Nakagawa, Atsuhiro; Oguchi, Yusuke; Oikawa, Minoru; Ota, Tadataka; Shiba, Kiyotaka; Shintaku, Hirofumi; Shirasaki, Yoshitaka; Suga, Kanako; Suzuki, Yuta; Suzuki, Nobutake; Tanaka, Yo; Tezuka, Hiroshi; Toyokawa, Chihana; Yalikun, Yaxiaer; Yamada, Makoto; Yamagishi, Mai; Yamano, Takashi; Yasumoto, Atsushi; Yatomi, Yutaka; Yazawa, Masayuki; Di Carlo, Dino; Hosokawa, Yoichiroh; Uemura, Sotaro; Ozeki, Yasuyuki.
Afiliação
  • Nitta N; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Japan Science and Technology Agency, Saitama 332-0012, Japan.
  • Sugimura T; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Japan Science and Technology Agency, Saitama 332-0012, Japan.
  • Isozaki A; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Mikami H; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Hiraki K; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Sakuma S; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603, Japan.
  • Iino T; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.
  • Arai F; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603, Japan.
  • Endo T; Center for Advanced Intelligence Project, RIKEN, Tokyo 103-0027, Japan; ExaWizards Inc., Tokyo 105-0013, Japan.
  • Fujiwaki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.
  • Fukuzawa H; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
  • Hase M; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Hayakawa T; Department of Precision Mechanics, Chuo University, Tokyo 112-8551, Japan.
  • Hiramatsu K; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Hoshino Y; Department of Chemical Engineering, Kyushu University, Fukuoka 819-0395, Japan.
  • Inaba M; Department of Creative Informatics, The University of Tokyo, Tokyo 113-0033, Japan.
  • Ito T; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Japan Science and Technology Agency, Saitama 332-0012, Japan.
  • Karakawa H; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Kasai Y; Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603, Japan.
  • Koizumi K; Department of Creative Informatics, The University of Tokyo, Tokyo 113-0033, Japan.
  • Lee S; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Lei C; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Li M; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Maeno T; Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
  • Matsusaka S; Department of Gastroenterology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan.
  • Murakami D; Department of Creative Informatics, The University of Tokyo, Tokyo 113-0033, Japan.
  • Nakagawa A; Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8577, Japan.
  • Oguchi Y; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Oikawa M; Science and Technology Unit, Natural Sciences Cluster, Kochi University, Kochi 780-8520, Japan.
  • Ota T; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
  • Shiba K; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan.
  • Shintaku H; Department of Micro Engineering, Kyoto University, Kyoto 606-8501, Japan.
  • Shirasaki Y; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Suga K; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan.
  • Suzuki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.
  • Suzuki N; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Tanaka Y; Center for Biosystems Dynamics Research, RIKEN, Osaka 565-0871, Japan.
  • Tezuka H; Department of Creative Informatics, The University of Tokyo, Tokyo 113-0033, Japan.
  • Toyokawa C; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
  • Yalikun Y; Center for Biosystems Dynamics Research, RIKEN, Osaka 565-0871, Japan.
  • Yamada M; Center for Advanced Intelligence Project, RIKEN, Tokyo 103-0027, Japan; Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan.
  • Yamagishi M; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Yamano T; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
  • Yasumoto A; Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Yatomi Y; Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Yazawa M; Department of Rehabilitation and Regenerative Medicine, Pharmacology, Columbia University, New York, NY 10032, USA.
  • Di Carlo D; Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Mechanical Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; California NanoSystems In
  • Hosokawa Y; Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
  • Uemura S; Department of Biological Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Ozeki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.
Cell ; 175(1): 266-276.e13, 2018 09 20.
Article em En | MEDLINE | ID: mdl-30166209
ABSTRACT
A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Ensaios de Triagem em Larga Escala / Citometria de Fluxo Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Ensaios de Triagem em Larga Escala / Citometria de Fluxo Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article