Evaluation of rapid KPC carbapenemase detection method based on MALDI-TOF VITEK MS spectra analysis.
J Med Microbiol
; 67(10): 1474-1479, 2018 Oct.
Article
em En
| MEDLINE
| ID: mdl-30168794
ABSTRACT
Clinical microbiology laboratories in hospital settings need to be able to identify patients who carry carbapenemase-producing bacterial strains quickly in order to contain their spread and initiate proper pharmacological therapy. The aim of this study was to confirm the correlation between KPC production and a characteristic mass spectrometry (MS) peak (11â109 Da±8) to validate the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS as a rapid screening tool. With this aim, 176 selected clinical samples that were KPC-producing and 260 control samples that were carbapenem-susceptible or carbapenem-resistant through other resistance mechanisms, or were producing hydrolytic enzymes other than KPC, were analysed. The presence of the 11â109 Da peak in the spectra of 99.4â% (175/176) of the KPC-producing strains compared to the controls, which all lacked the peak, confirmed a strong correlation between KPC production and the presence of the 11â109 Da peak in the MALDI-TOF MS spectrum. The high sensitivity (98.7â%) and specificity (100â%) of the peak searching in the MALDI-TOF MS spectra mean that 11â109 Da peak searching is a suitable screening tool in KPC-endemic regions.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Beta-Lactamases
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Carbapenêmicos
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Enterobacteriaceae
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Infecções por Enterobacteriaceae
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Antibacterianos
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article