Ca2+ Signals in Astrocytes Facilitate Spread of Epileptiform Activity.
Cereb Cortex
; 28(11): 4036-4048, 2018 11 01.
Article
em En
| MEDLINE
| ID: mdl-30169757
ABSTRACT
Epileptic seizures are associated with increased astrocytic Ca2+ signaling, but the fine spatiotemporal kinetics of the ictal astrocyte-neuron interplay remains elusive. By using 2-photon imaging of awake head-fixed mice with chronic hippocampal windows we demonstrate that astrocytic Ca2+ signals precede neuronal Ca2+ elevations during the initial bout of kainate-induced seizures. On average, astrocytic Ca2+ elevations preceded neuronal activity in CA1 by about 8 s. In subsequent bouts of epileptic seizures, astrocytes and neurons were activated simultaneously. The initial astrocytic Ca2+ elevation was abolished in mice lacking the type 2 inositol-1,4,5-trisphosphate-receptor (Itpr2-/-). Furthermore, we found that Itpr2-/- mice exhibited 60% less epileptiform activity compared with wild-type mice when assessed by telemetric EEG monitoring. In both genotypes we also demonstrate that spreading depression waves may play a part in seizure termination. Our findings imply a role for astrocytic Ca2+ signals in ictogenesis.
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Base de dados:
MEDLINE
Assunto principal:
Convulsões
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Astrócitos
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Sinalização do Cálcio
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Epilepsia
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Hipocampo
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article