[Asthenozoospermia is not correlated with 3' UTR polymorphisms of the GRP78 gene].
Zhonghua Nan Ke Xue
; 24(7): 596-601, 2018 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-30173441
ABSTRACT
OBJECTIVE:
To investigate the correlation of the single nucleotide polymorphisms (SNPs) rs12009, rs1140763 and rs16927997 in the 3'-untranslated region (3'UTR) of the glucose-regulated protein 78 (GRP78) gene with the risk of male asthenozoospermia (AZS).METHODS:
We included 400 AZS patients in the AZS group and another 400 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs12009, rs1140763 and rs16927997 polymorphisms in the 3'UTR of the GRP78 gene in all the male subjects and analyzed the association of the three SNPs with AZS.RESULTS:
The percentage of progressively motile sperm was significantly lower in the AZS group than in the normal controls (ï¼»20.09 ± 8.18ï¼½ % vs ï¼»57.16 ± 13.45ï¼½ %, P <0.01). Three genotypes of CC, CT and TT and 2 alleles of C and T were found in rs12009 and rs1140763 of the GRP78 gene, and another three genotypes of GG, GA and AA and two alleles of G and A were observed in rs16927997. There were no statistically significant differences between the control and AZS groups in the frequencies of the C and T alleles in rs12009 (44.3% vs 47.3% and 55.7% vs 52.7%, P >0.05) or rs1140763 (50.0% vs 52.0% and 50.0% vs 48.0%, P >0.05) or those of the G and A alleles in rs16927997 (6.0% vs 4.4% and 94.0% vs 95.6%, P >0.05), nor in the genotypes and allele frequencies of the 3 polymorphisms (P >0.05). Furthermore, three haplotypes of C-C-A, T-C-G and T-T-A were observed in the male subjects but showed no evident correlation between the AZS and normal control groups.CONCLUSIONS:
The polymorphisms in the 3'UTR of the GRP78 gene are not correlated with the risk of male asthenozoospermia.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Regiões 3' não Traduzidas
/
Polimorfismo de Nucleotídeo Único
/
Astenozoospermia
/
Proteínas de Choque Térmico
Tipo de estudo:
Etiology_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article